[Preparation of raw materials] The cake was crushed through an 8-mesh sieve and used. Purchase seed, cellulolytic enzyme, yeast, amylase saccharification enzyme, sulfuric acid (or any one of hydrochloric acid, phosphoric acid and acetic acid), alcohol, sodium hydroxide, ammonium hydrogen phosphate, urea for use.
[Product properties and uses] Rice bran protein products are yellowish brown with a protein content of 94% to 99%; yeast protein feed products are rich in crude protein; both products have good amino acid composition, easy to digest, and good color and taste. Rice bran protein is available for human consumption or as a raw material for food. Yeast protein feed can be used to raise poultry, livestock and fish.
[Production equipment and instruments] Pressure cooker, fermenter, 8 mesh sieve, centrifuge, mixer, dryer, thermometer.
[Process flow] standby material → extraction → washing → separation → drying → rice bran protein.
Rice husk → sulfuric acid → hydrolysis → neutralization → fermentation → drying → yeast feed finished product.
[Operation Steps]
(1) Production of rice bran protein An appropriate amount of water and a 5% 0.05% sodium hydroxide solution of cerium powder were added to the standby material, and the mixture was stirred at 37 ° C for 2 hours, and the residue was separated by a centrifuge. The extract is kept below 60 ° C, and the pH is adjusted to 4.5 to 5.0 with sulfuric acid (or any of hydrochloric acid, phosphoric acid, and acetic acid). At this pH, the solubility of the protein is the smallest, and it is coagulated and precipitated from the solution. The precipitated rice bran protein was washed with 85% alcohol (about 1:25), stirred at room temperature for 1 hour, and then the impurities were separated by a centrifuge, and the residue was dried to obtain rice bran protein.
(2) Protein feed production 100 kg of rice husk raw material powder, 5 kg of urea, 2 to 5 kg of sulfuric acid, 50 kg of water, 2 kg of yeast, and 250 g of amylase saccharification enzyme.
Slowly pour sulfuric acid into 50kg of water, then add 100kg of rice husk, stir it evenly in the pot, hydrolyze for 60-90min under steam pressure 1.4MPa, and obtain a sugar aqueous solution containing 1.7%~2% of reducing sugar. The mixture is placed in a fermenter with lime water, and the material is placed in a fermenter for 10 hours to obtain a mature yeast mash for drying, which is a yeast feed product.
Or take 100kg of raw rice husk powder, 20kg of rice bran, 3kg of ammonium hydrogen phosphate, 40kg of water, 50g of koji (wine), 100g of cellulolytic enzyme, mix well, ferment for 48h at 30 °C, you can get 145kg Fermentation of high protein feed.
[Notes]
(1) Processing of rice bran protein must be approved by the relevant state authorities to handle relevant procedures. The processing owner must have a hygiene permit, and the practitioner must conduct regular health checks. The quality of the product must meet the food standard. The pharmaceutical reagents used should be of chemical grade.
(2) Sulfuric acid, hydrochloric acid and sodium hydroxide are strong acids and alkalis. Wear protective clothing, gloves and masks during operation to prevent acid and alkali burns. Alcohol is a volatile, flammable chemical that bans open flames on the spot. The disposal and discharge of waste liquid must comply with relevant national regulations to prevent environmental pollution.
(3) When sulfuric acid is diluted, only sulfuric acid can be slowly added to the water. Never add water to sulfuric acid. Once water is added to the sulfuric acid, the sulfuric acid immediately "boiling" and splashing around, easily causing sulfuric acid burns. Good feed
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